Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection

نویسندگان

  • Arvind Chandrasekaran
  • Ashwin Acharya
  • Jian Liang You
  • Kim Young Soo
  • Muthukumaran Packirisamy
  • Ion Stiharu
  • Andre Darveau
چکیده

The desideratum to develop a fully integrated Lab-on-a-chip device capable ofrapid specimen detection for high throughput in-situ biomedical diagnoses and Point-of-Care testing applications has called for the integration of some of the novel technologiessuch as the microfluidics, microphotonics, immunoproteomics and Micro ElectroMechanical Systems (MEMS). In the present work, a silicon based microfluidic device hasbeen developed for carrying out fluorescence based immunoassay. By hybrid attachment ofthe microfluidic device with a Spectrometer-on-chip, the feasibility of synthesizing anintegrated Lab-on-a-chip type device for fluorescence based biosensing has beendemonstrated. Biodetection using the microfluidic device has been carried out usingantigen sheep IgG and Alexafluor-647 tagged antibody particles and the experimentalresults prove that silicon is a compatible material for the present application given thevarious advantages it offers such as cost-effectiveness, ease of bulk microfabrication,superior surface affinity to biomolecules, ease of disposability of the device etc., and is thussuitable for fabricating Lab-on-a-chip type devices.

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عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2007